Vassalli J D, Hamilton J, Reich E
Cell. 1977 Jul;11(3):695-705. doi: 10.1016/0092-8674(77)90086-1.
The synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin A and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting cAMP metabolism. By the manipulation of stimulatory and inhibitory influences, enzyme production can be modulated continuously over a 200 fold range. In the same way, the proportion of cells that secrete detectable levels of enzyme can be varied from 1-90%. No comparable modulation of lysozyme or acid hydrolase production is observed under the same conditions. These results suggest that the physiological control of macrophage plasminogen activator production is achieved by the interacting effects of mutually antagonistic stimuli; this emphasizes the utility of this enzyme for the study of regulatory phenomena, including those relating to inflammation.
培养的巨噬细胞产生和分泌纤溶酶原激活物可被刀豆球蛋白A和十四酰佛波醇乙酸酯诱导和刺激，并被糖皮质激素、有丝分裂抑制剂以及影响环磷酸腺苷（cAMP）代谢的化合物等试剂所抑制。通过对刺激和抑制作用的调控，酶的产生可在200倍的范围内持续调节。同样，分泌可检测水平酶的细胞比例可在1%至90%之间变化。在相同条件下，未观察到溶菌酶或酸性水解酶产生的类似调节。这些结果表明，巨噬细胞纤溶酶原激活物产生的生理控制是通过相互拮抗刺激的相互作用实现的；这强调了这种酶在研究调节现象（包括与炎症相关的现象）中的实用性。
