Pawlicki L, Zasłonka J, Kedziora J, Błaszczyk J, Sibińska E, Mussur M, Jaszewski R, Iwaszkiewicz A, Golański R, Szymańska E, Stefaniak B
I Kliniki Chorób Wewnetrznych IMW WAM w Lodzi.
Pol Arch Med Wewn. 1996 May;95(5):443-52.
The number of granulocytes, their capability to generate O2-. and the activity of SOD-1, GSH-Px, Cat as well as MDA concentrations in erythrocytes in the blood extracted from the venous sinus and aorta under coronary artery bypass with use of St. Thomas cardioplegic solution were determined. The blood for examination was obtained before the institution of cardiopulmonary bypass, in the period of the deepest ischaemia (just after declamping of the aorta) and between the 1-3 minute and the 10-13 minute of reperfusion. A rise in the number of granulocytes both in the venous sinus and aortal blood at all examined intervals was noted. Capability to produce superoxide anion radicals decreased at the peak of ischemia and during reperfusion. The activity of SOD-1 was lower both after the period of ischemia and reperfusion. A rise in aortal blood activity during reperfusion was characteristic for GSH-Px; the activity was greater in the blood sampled from the coronary sinus during ischemia and initial reperfusion. With the exception of the initial reperfusion the activity of Cat diminished in all observed cases. We did not observe any significant changes in MDA concentration with the exception of the initial reperfusion in the aortal blood and later during reperfusion in the blood from the coronary sinus. The results demonstrate that the applied cardioplegic solution may protect myocardium from harmful effects of active oxygen froms produced as a results of ischemia and reperfusion.
测定了在使用圣托马斯心脏停搏液进行冠状动脉搭桥手术时，从静脉窦和主动脉抽取的血液中粒细胞数量、其产生O2-的能力以及红细胞中SOD-1、GSH-Px、Cat的活性和MDA浓度。检查用血在体外循环建立前、最深缺血期（主动脉夹闭刚放开后）以及再灌注1 - 3分钟和10 - 13分钟时获取。在所有检查时间点，静脉窦和主动脉血液中的粒细胞数量均有增加。超氧阴离子自由基产生能力在缺血高峰期和再灌注期间降低。缺血期和再灌注后SOD-1的活性均降低。再灌注期间主动脉血液中GSH-Px活性升高；在缺血期和初始再灌注期间，从冠状窦采集的血液中该活性更高。除了初始再灌注外，在所有观察病例中Cat活性均降低。除了主动脉血液的初始再灌注以及后来冠状窦血液再灌注期间，我们未观察到MDA浓度有任何显著变化。结果表明，所应用的心脏停搏液可保护心肌免受缺血和再灌注产生的活性氧形式的有害影响。
