Maxwell W M, Robinson S J, Roca J, Molinia F C, Sánchez-Partida L G, Evans G
Department of Animal Science, University of Sydney, NSW, Australia.
Reprod Fertil Dev. 1995;7(5):1081-7. doi: 10.1071/rd9951081.
The motility, acrosome integrity and fertility of ram spermatozoa were examined after treatment with five compounds (caffeine, pentoxifylline, cAMP, 2-deoxyadenosine and kallikrein). Semen was extended with a Tris-based medium and frozen in pellet form. The compounds were added at various concentrations to the semen before freezing or after thawing. Motility and acrosome characteristics were assessed over a 6-h post-thaw period of incubation at 37 degrees C. Of the five compounds examined, only pentoxifylline, when added after thawing, stimulated motility but had not effect on the acrosome integrity of spermatozoa. Pregnancy rates for ewes inseminated in the uterus with semen treated after thawing with Dulbecco's phosphate-buffered saline (PBS, control) or PBS containing pentoxifylline (15 mM in the thawed semen), were 16/39 (41%) and 22/42 (52%) respectively. Motility characteristics of spermatozoa assessed by image analysis (Hamilton-Thorne analyser) were initially better after treatment of thawed semen with pentoxifylline than with PBS ("percent motility', "percent progressive motility', "percent rapid', "percent moderate' and "percent static', P < 0.01 or P < 0.001), but there was a greater deterioration in these characteristics during post-thaw incubation in semen treated with pentoxifylline than in the controls. The deterioration in motility characteristics occurred within 4 h of thawing and this may have been responsible for the modest improvement in fertility of spermatozoa treated with pentoxifylline.
用五种化合物（咖啡因、己酮可可碱、环磷酸腺苷、2-脱氧腺苷和激肽释放酶）处理后，检测了公羊精子的活力、顶体完整性和受精能力。精液用基于Tris的培养基稀释并制成颗粒冷冻保存。在冷冻前或解冻后，将不同浓度的化合物添加到精液中。在37℃解冻后孵育6小时的时间段内评估精子的活力和顶体特征。在所检测的五种化合物中，只有己酮可可碱在解冻后添加时能刺激精子活力，但对精子顶体完整性没有影响。用杜尔贝科磷酸盐缓冲盐水（PBS，对照）或含己酮可可碱（解冻精液中为15 mM）的PBS解冻后处理的精液对母羊进行子宫内授精，妊娠率分别为16/39（41%）和22/42（52%）。通过图像分析（汉密尔顿-桑恩分析仪）评估，解冻精液用己酮可可碱处理后的精子活力特征最初比用PBS处理的更好（“活力百分比”、“前向运动百分比”、“快速运动百分比”、“中等运动百分比”和“静止百分比”，P<0.01或P<0.001），但己酮可可碱处理的精液在解冻后孵育期间这些特征的恶化程度比对照组更大。活力特征的恶化在解冻后4小时内发生，这可能是己酮可可碱处理的精子受精能力适度提高的原因。
