Kiang S W, Kuan J W, Kuan S S, Guilbault G G
Clin Chim Acta. 1977 Aug 1;78(3):495-8. doi: 10.1016/0009-8981(77)90083-3.
We have adapted the hexokinase glucose procedure to an immobilized enzyme stirrer for the determination of glucose concentrations in human blood plasma. The procedure is a fluorometric rate method measuring the formation of NADPH catalyzed by immobilized glucose-6-phosphate dehydrogenase and hexokinase held within a tiny stirrer. The enzyme stirrer is stable for at least two months and can be used over eight-hundred assays without any loss of activity.
