Rosenzweig Z, Kopelman R
Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.
Anal Chem. 1995 Aug 1;67(15):2650-4. doi: 10.1021/ac00111a024.
A submicrometer optical fiber oxygen sensor has been fabricated, based on the fluorescence quenching of tris-(1,10-phenanthroline)ruthenium(II) chloride in the presence of oxygen or dissolved oxygen. The Ru compound has been incorporated into acrylamide polymer that is attached covalently to a silanized optical fiber tip surface by photoinitiated polymerization. Leaching of the sensing reagent from the polymer host matrix has been minimized by the optimization of the ratio between the acrylamide monomer and the cross-linker, N,N-methylenebisacrylamide. The sensor is fully reversible and highly reproducible. A standard deviation of approximately 2% for 10 consecutive fluorescence measurements has been observed for several oxygen concentrations. The sample volume required for measurements is 100 fL. An absolute detection limit of 1 x 10(-17) mol is achieved. This is an improvement by a factor of 10(6) as compared to other existing optical fiber oxygen sensors.
基于氯化三（1,10-菲咯啉）钌（II）在氧气或溶解氧存在下的荧光猝灭，制备了一种亚微米级光纤氧传感器。通过光引发聚合将钌化合物掺入丙烯酰胺聚合物中，该聚合物通过共价键连接到硅烷化光纤尖端表面。通过优化丙烯酰胺单体与交联剂N,N-亚甲基双丙烯酰胺之间的比例，使传感试剂从聚合物主体基质中的浸出最小化。该传感器具有完全可逆性和高重现性。在几种氧浓度下，连续10次荧光测量的标准偏差约为2%。测量所需的样品体积为100 fL。实现了1×10⁻¹⁷ mol的绝对检测限。与其他现有的光纤氧传感器相比，这提高了10⁶倍。
