Yao Y Q, Ng V, Yeung W S, Ho P C
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
Andrologia. 1996 Mar-Apr;28(2):127-31. doi: 10.1111/j.1439-0272.1996.tb02768.x.
Morphology and motility are important parameters for assessing the fertilizing capacity of spermatozoa. This investigation reports a systematic study on the profiles of these parameters after percoll gradient centrifugation. Spermatozoa from normal human semen were fractionated by discontinuous Percoll gradients (30%, 45%, 75%, 90%). Spermatozoa washed with Earle's balanced salt solution were used as a control. After centrifugation, sperm morphology was evaluated according to strict criteria; motility was assessed by a computer-assisted semen analysis system. The results showed that the percentage of spermatozoa with normal morphology increased, while those with severe morphological defects decreased, as the density of Percoll increased. The percentages of motile spermatozoa and hyperactivated spermatozoa, and the velocity and amplitude of lateral head displacement of the spermatozoa were significantly higher in the 75% and 90% Percoll fractions than that in the 30% and 45% ones, and in the control. These results demonstrated that Percoll density gradient centrifugation enriched spermatozoa in terms of morphology and motility.
形态和活力是评估精子受精能力的重要参数。本研究报告了对经Percoll梯度离心后这些参数概况的系统研究。通过不连续Percoll梯度（30%、45%、75%、90%）对正常人精液中的精子进行分级分离。用Earle平衡盐溶液洗涤的精子用作对照。离心后，根据严格标准评估精子形态；通过计算机辅助精液分析系统评估活力。结果显示，随着Percoll密度增加，形态正常的精子百分比增加，而存在严重形态缺陷的精子百分比降低。在75%和90%Percoll组分中，活动精子和超活化精子的百分比以及精子头部侧向位移的速度和幅度显著高于30%和45%组分以及对照中的相应值。这些结果表明，Percoll密度梯度离心在形态和活力方面富集了精子。
