定位影响秀丽隐杆线虫生活史性状的数量性状基因座。
Mapping quantitative trait loci affecting life history traits in the nematode Caenorhabditis elegans.
作者信息
Shook D R, Brooks A, Johnson T E
机构信息
Institute for Behavioral Genetics, University of Colorado, Boulder 80309, USA.
出版信息
Genetics. 1996 Mar;142(3):801-17. doi: 10.1093/genetics/142.3.801.
We have identified chromosomal regions containing quantitative trait loci (QTLs) specifying life history traits in recombinant-inbred strains of the nematode Caenorhabditis elegans. This approach also allows us to examine epistatic interactions between loci and pleiotropic effects on different traits at specific loci. QTLs for mean life span were identified on chromosomes II (near stP101), IV (stP5) and the X (stP61), and QTLs for fertility were identified on II (maPI), III (stP19) and IV (stP51). The QTLs for mean life span accounted for 90% of the genetic component of variance. The loci for mean fertility accounted for 88% of the genetic component of variance. Additional QTLs for temperature-sensitive fertility [II (stP36) and V (stP6)] and internal hatching [IV (stP5)] were also mapped in these crosses. We found evidence for epistatic effects on mean life span between maP1 and bP1 (V), and for epistatic effects on mean fertility between stP36 and stP6, between stP98 (II) and stP192 (V), between maP1 and stP127 (III), between maP1 and stP103 (X), and between stP5 and stP6. Negatively correlated, pleiotropic effects on mean life span and internal hatching were found linked to stP5.
我们已经在秀丽隐杆线虫的重组自交系中鉴定出了包含数量性状基因座（QTL）的染色体区域，这些基因座决定了生活史性状。这种方法还使我们能够研究基因座之间的上位性相互作用以及特定基因座对不同性状的多效性影响。在染色体II（靠近stP101）、IV（stP5）和X（stP61）上鉴定出了平均寿命的QTL，在II（maPI）、III（stP19）和IV（stP51）上鉴定出了生育力的QTL。平均寿命的QTL占遗传方差成分的90%。平均生育力的基因座占遗传方差成分的88%。在这些杂交实验中还定位了温度敏感性生育力[II（stP36）和V（stP6）]以及内部孵化[IV（stP5）]的其他QTL。我们发现了maP1和bP1（V）之间对平均寿命有上位性影响的证据，以及stP36和stP6之间、stP98（II）和stP192（V）之间、maP1和stP127（III）之间、maP1和stP103（X）之间以及stP5和stP6之间对平均生育力有上位性影响的证据。发现与stP5相关的对平均寿命和内部孵化呈负相关的多效性影响。
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