Jaffe B H, Walsh J T
Biomedical Engineering Department, Northwestern University, Evanston, Illinois 60208, USA.
Lasers Surg Med. 1996;18(1):1-9. doi: 10.1002/(SICI)1096-9101(1996)18:1<1::AID-LSM1>3.0.CO;2-W.
The clinical use of lasers to cut and coagulate tissue necessitates a better understanding of how the residual thermal damage affects healing. This study was designed to evaluate the effects of varying degrees of thermal damage on the healing process.
STUDY DESIGN, MATERIALS AND METHODS: Partial-thickness lesions were created in guinea pig skin using an Er:YAG laser, a Ho:YAG laser, and a scalpel. To monitor recovery of the stratum corneum, water flux from the wound sites was quantified and histological data obtained for approximately one week.
The data indicate an exponential water loss pattern from all wounds. Water flux from the scalpel- and the Er:YAG laser-induced wounds was initially high but decreased rapidly with decay rates (mean +/- SE) of 0.46 +/- 0.01 day-1 and 0.38 +/- 0.01 day-1, respectively. The Ho:YAG laser-induced wounds demonstrated a different pattern of decay with lower water flux values initially and a decay rate of only 0.13 +/- 0.01 day-1.
Histological and water flux data reveal that Er:YAG laser-induced wounds achieve epidermal integrity only slightly after scalpel-induced lesions, and Ho:YAG laser-induced wounds heal substantially slower and contain more granulation tissue.
临床使用激光切割和凝固组织需要更好地了解残余热损伤如何影响愈合。本研究旨在评估不同程度的热损伤对愈合过程的影响。
研究设计、材料与方法：使用铒：钇铝石榴石激光、钬：钇铝石榴石激光和手术刀在豚鼠皮肤上制造部分厚度的损伤。为监测角质层的恢复情况，对伤口部位的水通量进行量化，并获取约一周的组织学数据。
数据表明所有伤口均呈现指数形式的水分流失模式。手术刀和铒：钇铝石榴石激光所致伤口的水通量最初较高，但分别以0.46±0.01天⁻¹和0.38±0.01天⁻¹的衰减率迅速下降。钬：钇铝石榴石激光所致伤口呈现不同的衰减模式，最初水通量值较低，衰减率仅为0.13±0.01天⁻¹。
组织学和水通量数据显示，铒：钇铝石榴石激光所致伤口在手术刀所致损伤后仅略微实现表皮完整性，而钬：钇铝石榴石激光所致伤口愈合明显较慢且含有更多肉芽组织。
