van Loveren C, Buijs J F, Buijs M J, ten Cate J M
Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdm (ACTA), Amsterdam, Netherlands.
Caries Res. 1996;30(1):45-51. doi: 10.1159/000262136.
In an in vitro demineralization model, the protective effect of two chlorhexidine varnishes, Cervitec (1% w/w chlorhexidine diacetate and 1% w/w thymol) and EC40 (40% w/w chlorhexidine diacetate), was compared with that of Fluor Protector, a varnish containing 0.1% w/w F. The demineralization model comprised an acidogenic Streptococcus mutans suspension in agarose placed on enamel or dentine specimens. The experiments extended over three serial 22-hour demineralization periods with fresh S. mutans suspensions for each period. To determine whether the varnishes released enough demineralization-inhibiting compounds, approximately 10 microliters of the varnishes was applied adjacent to the enamel and dentine specimens just before the first application of the S. mutans suspensions and left during the serial experiments (release study). In a separate series of experiments, the effect of the pretreatment of the enamal and dentine specimens with the various varnishes was tested (pretreatment study). In the release study the protective effect for enamel decreased in the order: EC40 = Fluor Protector >> Cervitec = no treatment. For dentine this order was: EC40 >> Fluor Protector = Cervitec > no treatment. In the pretreatment study, the enamel specimens were best protected by Fluor Protector (Fluor Protector >> Cervitec = EC40 > no treatment), while the dentine specimens were best protected by the chlorhexidine treatments (Cervitec = EC40 > Fluor Protector > no treatment). A 1:1 mixture of Cervitec and Fluor Protector was as effective as the most effective component alone. It is concluded that a varnish containing both fluoride and chlorhexidine may be useful, since it could give optimal protection to both enamel and dentine.
在体外脱矿模型中，将两种洗必泰清漆Cervitec（1% w/w二醋酸氯己定和1% w/w百里酚）和EC40（40% w/w二醋酸氯己定）的保护效果与含0.1% w/w氟的清漆氟保护漆进行了比较。脱矿模型包括置于牙釉质或牙本质标本上的琼脂糖中产酸变形链球菌悬液。实验持续三个连续的22小时脱矿期，每个时期使用新鲜的变形链球菌悬液。为了确定清漆是否释放出足够的抑制脱矿化合物，在首次应用变形链球菌悬液之前，将约10微升清漆涂在牙釉质和牙本质标本旁边，并在系列实验过程中保留（释放研究）。在另一系列实验中，测试了用各种清漆对牙釉质和牙本质标本进行预处理的效果（预处理研究）。在释放研究中，对牙釉质的保护效果顺序为：EC40 = 氟保护漆 >> Cervitec = 未处理。对于牙本质，顺序为：EC40 >> 氟保护漆 = Cervitec > 未处理。在预处理研究中，牙釉质标本受氟保护漆保护最佳（氟保护漆 >> Cervitec = EC40 > 未处理），而牙本质标本受洗必泰处理保护最佳（Cervitec = EC40 > 氟保护漆 > 未处理）。Cervitec和氟保护漆的1:1混合物与单独最有效的成分效果相同。结论是，含氟和洗必泰的清漆可能有用，因为它可以对牙釉质和牙本质都提供最佳保护。
