State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics & Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Department of Operative Dentistry and Endodontics & Stomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Biomed Res Int. 2019 Jan 21;2019:6393470. doi: 10.1155/2019/6393470. eCollection 2019.
Quaternary ammonium compounds constitute a large group of antibacterial chemicals with a potential for inhibiting dental plaque. The aims of this study were to evaluate short-time antibacterial and regulating effects of dimethylaminododecyl methacrylate (DMADDM) on multispecies biofilm viability, reformation, and bacterial composition in vitro. DMADDM, chlorhexidine (CHX), and sodium fluoride (NaF) were chosen in the present study. , and were used to form multispecies biofilm. Cytotoxicity assay was used to determine the optimal tested concentration. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and resazurin test of biofilm were conducted to study the biomass changes and metabolic changes of controlled multispecies biofilm. Scanning electron microscopy (SEM) was used to observe biofilm images. TaqMan real-time polymerase chain reaction was performed to evaluate the proportion change in multispecies biofilm of different groups. Cytotoxicity assay showed that there existed a certain concentration application range for DMADDM, CHX, and NaF. MTT assay and resazurin test results showed that DMADDM and CHX groups decreased multispecies biofilm growth and metabolic activity ( < 0.05), no matter after 1 min or 5 min direct contact killing or after 24 h regrowth. The proportion of decreased steadily in DMADDM and CHX groups after 1 min and 5 min direct contact killing and 24 h regrowth, compared to control groups. A novel DMADDM-containing solution was developed, achieving effective short-time antibacterial effects and regulation ability of biofilm formation.
季铵盐化合物构成了具有抑制牙菌斑潜力的一大类抗菌化学物质。本研究旨在评估二甲氨基十二烷基甲基丙烯酸酯（DMADDM）对体外多种生物膜活力、再形成和细菌组成的短期抗菌和调节作用。在本研究中选择了 DMADDM、洗必泰（CHX）和氟化钠（NaF）。 、 和 用于形成多种生物膜。细胞毒性测定用于确定最佳测试浓度。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物（MTT）测定和生物膜的 Resazurin 试验用于研究受控多种生物膜的生物量变化和代谢变化。扫描电子显微镜（SEM）用于观察生物膜图像。TaqMan 实时聚合酶链反应用于评估不同组别的多种生物膜比例变化。细胞毒性测定表明，DMADDM、CHX 和 NaF 存在一定的浓度应用范围。MTT 测定和 Resazurin 试验结果表明，DMADDM 和 CHX 组均降低了多种生物膜的生长和代谢活性（<0.05），无论是 1 分钟还是 5 分钟直接接触杀伤后，还是 24 小时再生长后。与对照组相比，DMADDM 和 CHX 组在 1 分钟和 5 分钟直接接触杀伤以及 24 小时再生长后， 的比例稳定下降。开发了一种新型的含 DMADDM 溶液，实现了有效的短期抗菌效果和生物膜形成的调节能力。
