Yang S, Atanasov P, Wilkins E
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque 87131-1341, USA.
Biomed Instrum Technol. 1996 Jan-Feb;30(1):55-61.
An implantable biosensor for continuous monitoring of glucose levels in diabetic patients was developed. The biosensor is based on an amperometric oxygen electrode and glucose oxidase immobilized on carbon powder held in the form of a liquid suspension. The enzyme material can be replaced (the sensor recharged) without sensor disassembly. Sensors with polycarbonate glucose-diffusion membranes coated with silastic were studied. Calibration curves of the sensors in phosphate buffer solution and in undiluted blood plasma at body temperature were obtained and compared. Sensors were tested for long-term performance in vitro in model phosphate buffer solution and in blood plasma at room and at body temperature. Reproducibility of the sensor responses in plasma at body temperature was demonstrated. During continuous operation at body temperature, the sensors have a lifetime of at least six weeks.
开发了一种用于连续监测糖尿病患者血糖水平的可植入生物传感器。该生物传感器基于安培型氧电极和固定在以液体悬浮液形式保存的碳粉上的葡萄糖氧化酶。酶材料可以在不拆卸传感器的情况下进行更换（传感器充电）。研究了涂有硅橡胶的聚碳酸酯葡萄糖扩散膜的传感器。获得并比较了传感器在体温下的磷酸盐缓冲溶液和未稀释血浆中的校准曲线。在室温及体温下，在模型磷酸盐缓冲溶液和血浆中对传感器进行了体外长期性能测试。证明了传感器在体温下血浆中响应的可重复性。在体温下连续运行期间，传感器的寿命至少为六周。
