Xie S L, Wilkins E
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque.
J Biomed Eng. 1991 Sep;13(5):375-8. doi: 10.1016/0141-5425(91)90017-2.
A unique enzyme electrode was designed using glucose oxidase immobilized on fine graphite powder. The graphite-enzyme is in a fluid state which enables recharging of the system when the enzyme activity decreases, therefore allowing the system to have a long lifetime in a diabetic patient. The sensor was tested using glucose concentration in the range of 20-300 mg d-1. The electrode construction includes a hydrophobic membrane and a platinum electrode for detection of hydrogen peroxide. The signal output is large with minimal noise when tested in buffer. The sensor has been performing at ambient temperature for 4 months when stored overnight at 4 degrees C.
一种独特的酶电极是通过将葡萄糖氧化酶固定在精细石墨粉上设计而成的。石墨 - 酶处于流体状态，当酶活性降低时能够使系统再充电，因此该系统能在糖尿病患者体内拥有较长的使用寿命。使用浓度范围为20 - 300 mg d⁻¹的葡萄糖对该传感器进行了测试。电极结构包括一个疏水膜和一个用于检测过氧化氢的铂电极。在缓冲液中测试时，信号输出大且噪声最小。该传感器在环境温度下工作，当在4摄氏度下储存过夜时已持续运行4个月。
