Sutcliffe I C
Department of Oral Biology, University of Newcastle upon Tyne, U.K.
Arch Oral Biol. 1995 Dec;40(12):1119-24. doi: 10.1016/0003-9969(95)00086-0.
The oral organism Corynebacterium matruchotii was investigated for the presence of lipoteichoic acid, as this common polyanionic macroamphiphilic component of Gram-positive bacteria has been implicated in phenomena related to calcium binding. Phenol-water extraction followed by a small-scale, hydrophobic-interaction chromatography step yielded carbohydrate-containing preparations that were distinguished from lipoteichoic acid by their low phosphorus content. Subsequently, large-scale phenol-water extracts from each of three strains of C. matruchotii were purified by hydrophobic-interaction chromatography and shown to contain a heterogeneous lipoglycan fraction. The major fatty acids present were the same as for the whole-cell fatty acid profiles but differed in their relative amounts. Qualitative analysis of the lipoglycan fractions revealed similarities of carbohydrate composition with a previously characterized lipoglycan fraction from C. diphtheriae and with the lipoarabinomannan/lipomannans found in the genus Mycobacterium. The carbohydrate composition and the low phosphorus content indicated that lipoteichoic acid was absent from C. matruchotii. The calcium-binding properties of C. matruchotii therefore cannot be attributed to lipoteichoic acid.
对口腔微生物马氏棒状杆菌进行了脂磷壁酸检测，因为革兰氏阳性菌这种常见的聚阴离子大两亲性成分与钙结合相关现象有关。采用酚水萃取法，随后进行小规模疏水相互作用色谱步骤，得到了含碳水化合物的制剂，这些制剂因磷含量低而与脂磷壁酸不同。随后，通过疏水相互作用色谱法对三株马氏棒状杆菌的大规模酚水提取物进行了纯化，结果显示其含有不均一的脂多糖部分。主要脂肪酸与全细胞脂肪酸谱相同，但相对含量不同。对脂多糖部分的定性分析表明，其碳水化合物组成与先前鉴定的来自白喉棒状杆菌的脂多糖部分以及分枝杆菌属中的脂阿拉伯甘露聚糖/脂甘露聚糖相似。碳水化合物组成和低磷含量表明马氏棒状杆菌不存在脂磷壁酸。因此，马氏棒状杆菌的钙结合特性不能归因于脂磷壁酸。
