Fischer W
Eur J Biochem. 1987 Jun 15;165(3):639-46. doi: 10.1111/j.1432-1033.1987.tb11488.x.
The lipid macroamphiphile of Bifidobacterium bifidum subsp. pennsylvanicum DSM 20239 was extracted with phenol/water and purified by treatment with nucleases and hydrophobic interaction chromatography. From analytical data, the results of Smith degradation, hydrolysis with HF and methylation studies, the following structure is proposed: (formula; see text) where n and m are approximately 7-10 and 8-15, respectively. The monoglycerophosphate residues have the sn-glycero-1-phosphate configuration; 20-50% of them are substituted with L-alanine in ester linkage. The lipid anchor is most likely a galactosyldiacylglycerol, part of which carries a third fatty acid. This is the first example among gram-positive bacteria of a glycerophosphate-containing lipid macroamphiphile that carries the glycerophosphate residues as monomeric side chains on a lipoglycan. Further, it contains L-alanine in place of the D-alanine found in lipoteichoic acids.
两歧双歧杆菌宾夕法尼亚亚种DSM 20239的脂质大分子两亲物用苯酚/水提取，并通过核酸酶处理和疏水相互作用色谱法纯化。根据分析数据、史密斯降解结果、氢氟酸水解和甲基化研究，提出了以下结构：（分子式；见正文）其中n和m分别约为7-10和8-15。甘油磷酸单酯残基具有sn-甘油-1-磷酸构型；其中20-50%被L-丙氨酸以酯键取代。脂质锚最有可能是半乳糖基二酰基甘油，其中一部分带有第三个脂肪酸。这是革兰氏阳性细菌中第一个含有甘油磷酸的脂质大分子两亲物的例子，该两亲物在脂多糖上以单体侧链形式携带甘油磷酸残基。此外，它含有L-丙氨酸，取代了脂磷壁酸中的D-丙氨酸。
