Katoh K, Ishiguro S, Tamai M
Department of Ophthalmology, Tohoku University School of Medicine.
Nippon Ganka Gakkai Zasshi. 1996 Feb;100(2):145-9.
We attempted to purify endogenous growth factors of chick embryonal retinal pigment epithelial cells from bovine neural retina. Ammonium sulfate precipitation, gel filtration, and anion exchange column chromatography were used. Though only partially purification, bovine retinal extract had two effects, growth and transformation, on chick embryonal pigment epithelial cells. We think that these purified factors may be novel from the estimation of their molecular weight by gel filtration.
我们试图从牛神经视网膜中纯化鸡胚视网膜色素上皮细胞的内源性生长因子。采用了硫酸铵沉淀、凝胶过滤和阴离子交换柱色谱法。尽管只是部分纯化，但牛视网膜提取物对鸡胚色素上皮细胞有生长和转化两种作用。从凝胶过滤法估算的分子量来看，我们认为这些纯化因子可能是新的。
