Takabatake M
Department of Ophthalmology, Okayama University Medical School, Japan.
Nippon Ganka Gakkai Zasshi. 1997 Jan;101(1):30-9.
We observed the three dimensional structure of cellular aggregates formed from chick retinal cells in a floating culture system for 2 months. The aggregated cells partially mimicked the structure of the retina and showed differentiation of photoreceptor cells and Müller cells with numerous synapses. Immunohistochemical studies showed the number of anti-rhodopsin positive cells increasing over time. In the long-term culture, increasing anti-crystalline positive cells appeared late in the culture, indicative of differentiation of lens epithelial cells. Nerve, epidermal, and basic fibroblast growth factors, and co-culture with retinal pigment epithelial cells stimulated to some degree the growth of dendrites in retinal cellular aggregates. Epidermal growth factor, in particular, promoted the production of rhodopsin in photoreceptor cells. Retinal cellular aggregates in a floating culture system could be used to examine the effect of various factors on differentiation of the neuroretina.
我们在漂浮培养系统中观察了鸡视网膜细胞形成的细胞聚集体的三维结构，持续2个月。聚集的细胞部分模拟了视网膜的结构，并显示出光感受器细胞和穆勒细胞的分化以及大量突触。免疫组织化学研究表明，抗视紫红质阳性细胞的数量随时间增加。在长期培养中，抗晶状体阳性细胞数量增加，出现在培养后期，表明晶状体上皮细胞发生了分化。神经生长因子、表皮生长因子和碱性成纤维细胞生长因子，以及与视网膜色素上皮细胞共培养，在一定程度上刺激了视网膜细胞聚集体中树突的生长。特别是表皮生长因子促进了光感受器细胞中视紫红质的产生。漂浮培养系统中的视网膜细胞聚集体可用于研究各种因素对神经视网膜分化的影响。
