发育中大鼠大脑中的生长抑素受体

Somatostatin receptors in the developing rat brain.

作者信息

Thoss V S, Duc D, Hoyer D

机构信息

Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.

出版信息

Eur J Pharmacol. 1996 Feb 15;297(1-2):145-55. doi: 10.1016/0014-2999(95)00736-9.

DOI:10.1016/0014-2999(95)00736-9

PMID:8851178

Abstract

The distribution of [125I]SRIF-28 ([Leu8,D-Trp22,125I-Tyr25]somatostatin-28), [125I]204-090 ([Tyr3]octreotide) and [125I]CGP 23996 (c[Asu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Tyr-Thr-Ser]) labelled recognition sites was studied by autoradiography in rat brain at embryonic day 18 (E 18) and postnatal day 5 (P 5). These results were compared with mRNA expression of somatostatin receptors SSTR1-5 (named sst1-5 now) as studied by in situ hybridization. [125I]SRIF-28, [125I]204-090 and [125I]CGP 23996 binding displayed different although partially overlapping distributions, and showed an increase between E 18 and P 5, which was less marked for [125I]204-090 binding. -125I-204-090 binding and sst2 receptor mRNA were similarly distributed, whereas [125I]CGP 23996 binding did not correlate with any single somatostatin receptor mRNA. The data suggest that most SRIF receptor subtypes in rat brain are present before birth, but evolve differently.

摘要

采用放射自显影术研究了胚胎18天（E18）和出生后5天（P5）大鼠脑中[125I]SRIF-28（[Leu8，D-Trp22，125I-Tyr25]生长抑素-28）、[125I]204-090（[Tyr3]奥曲肽）和[125I]CGP 23996（c[Asu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Tyr-Thr-Ser]）标记的识别位点的分布。将这些结果与通过原位杂交研究的生长抑素受体SSTR1-5（现称为sst1-5）的mRNA表达进行比较。[125I]SRIF-28、[125I]204-090和[125I]CGP 23996的结合表现出不同但部分重叠的分布，并且在E18和P5之间有所增加，[125I]204-090结合的增加不太明显。-125I-204-090结合和sst2受体mRNA分布相似，而[125I]CGP 23996结合与任何单一生长抑素受体mRNA均无相关性。数据表明，大鼠脑中大多数SRIF受体亚型在出生前就已存在，但进化方式不同。