Thoss V S, Pérez J, Probst A, Hoyer D
Preclinical Research, SANDOZ Pharma Ltd., Basel, Switzerland.
Naunyn Schmiedebergs Arch Pharmacol. 1996 Oct;354(4):411-9. doi: 10.1007/BF00168430.
The messenger RNA (mRNA) expression of somatostatin receptors sst1-5 was studied in human brain by in situ hybridization histochemistry using specific oligonucleotide probes. sst1 receptor mRNA was mainly found in the outer and intermediate layers of cerebral cortex, hippocampal formation (CA1, dentate gyrus, entorhinal cortex), hypothalamus, substantia nigra, medullary nuclei and dentate nucleus. sst2 transcripts were present in the deep layers of the cerebral cortex, amygdala, hippocampal formation (CA1, dentate gyrus, subiculum, entorhinal cortex), the granular layer of the cerebellum and pituitary. sst3 receptor mRNA was localized in the cerebral cortex, hippocampal formation (CA1, dentate gyrus), several medullary nuclei and the granule and possibly Purkinje cell layer of the cerebellum and at very low levels in the pituitary. sst4 receptor mRNA was absent in the cerebral cortex. Intermediate signals were observed in the dentate gyrus of the hippocampus and several medullary nuclei while an intense expression was found in the granule and Purkinje cell layer of cerebellum. sst5 transcripts were present in the pituitary and the granule layer of the cerebellum. The present results show that mRNAs of sst1-4 somatostatin receptors have distinct distribution patterns within the human brain, although there is overlap in several regions. sst5 receptor mRNA expression appears to be very low and restricted to the cerebellum and pituitary. The distribution pattern observed in the human brain was broadly similar to that reported previously in the rat brain. The high expression levels of at least two somatostatin receptor subtype mRNAs (sst2 and sst5) in the pituitary gland suggest that somatostatin may affect neuroendocrine functions via more than one receptor.
采用特异性寡核苷酸探针，通过原位杂交组织化学技术研究了生长抑素受体sst1 - 5在人脑内的信使核糖核酸（mRNA）表达情况。sst1受体mRNA主要见于大脑皮质的外层和中层、海马结构（CA1、齿状回、内嗅皮质）、下丘脑、黑质、髓核和齿状核。sst2转录本存在于大脑皮质深层、杏仁核、海马结构（CA1、齿状回、海马下托、内嗅皮质）、小脑颗粒层和垂体。sst3受体mRNA定位于大脑皮质、海马结构（CA1、齿状回）、几个髓核以及小脑颗粒层和可能的浦肯野细胞层，在垂体中的表达水平很低。大脑皮质中未检测到sst4受体mRNA。在海马齿状回和几个髓核中观察到中等信号，而在小脑颗粒层和浦肯野细胞层中发现有强烈表达。sst5转录本存在于垂体和小脑颗粒层。目前的结果表明，尽管在几个区域存在重叠，但生长抑素受体sst1 - 4的mRNA在人脑内具有不同的分布模式。sst5受体mRNA表达似乎非常低，且局限于小脑和垂体。在人脑中观察到的分布模式与先前在大鼠脑中报道的大致相似。垂体中至少两种生长抑素受体亚型mRNA（sst2和sst5）的高表达水平表明，生长抑素可能通过不止一种受体影响神经内分泌功能。
