Janáky T, Szabó E, Baláspiri L, Adi B, Penke B
Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Szeged, Hungary.
J Chromatogr B Biomed Appl. 1996 Feb 9;676(1):7-12. doi: 10.1016/0378-4347(95)00402-5.
An analytical investigation of a new peptide family, the human galanins and their fragments, was carried out by reversed-phase HPLC, capillary zone electrophoresis (CZE) at different pH values and micellar electrokinetic capillary chromatography (MECC) in phosphate-borate-sodium dodecyl sulphate buffer. None of the methods seems to be superior to the others. The complementary nature of the electrophoretic methods is obvious when the profiles of peptides are compared; impurities not separated by HPLC are separated by CZE or MECC and vice versa. With these three different separation methods, a more complex analytical control of the synthetic work can be achieved.
通过反相高效液相色谱法、不同pH值下的毛细管区带电泳（CZE）以及在磷酸盐 - 硼酸盐 - 十二烷基硫酸钠缓冲液中的胶束电动毛细管色谱法（MECC），对一个新的肽家族——人甘丙肽及其片段进行了分析研究。似乎没有一种方法比其他方法更具优势。当比较肽的图谱时，电泳方法的互补性显而易见；未被高效液相色谱法分离的杂质可被毛细管区带电泳或胶束电动毛细管色谱法分离，反之亦然。使用这三种不同的分离方法，可以实现对合成工作更复杂的分析控制。
