Wimber D E, Wimber D R
Genetics. 1977 May;86(1):133-48. doi: 10.1093/genetics/86.1.133.
Drosophila melanogaster 125I-5S RNA was annealed to salivary gland preparations of 6 species in the virilis group of Drosophila. Two patterns of annealing were found. D. virilis, D. montana and D. borealis showed three 5S gene clusters on chromosome 5; Sd-f and Wc-j were strongly labeled, but Xa-e was weakly labeled. D. montana and D. borealis have a greater percentage of their total 5S cistroms at S d-f than does D. virilis. D. americana americana, D. americana texana and D. novamexicana showed 2 sites labeled; no label was seen at Sd-f while Wc-j was weakly labeled and Xa-e was strongly labeled, the reciprocal of the previous pattern in the W-X region. Hybrids between D. a americana and D. virilis showed no difference in chromosome banding at the sites of the 5S clusters despite their pattern difference. D. a texana X D. virilis, on the other hand, did show a difference in staining the Xa-e region. These patterns fall squarely into the biosystematic groupings deduced by many previous workers.
将果蝇黑腹果蝇的125I - 5S RNA与果蝇粗壮果蝇组6个物种的唾液腺制剂进行退火处理。发现了两种退火模式。粗壮果蝇、蒙大拿果蝇和北方果蝇在第5号染色体上显示出三个5S基因簇；Sd - f和Wc - j被强烈标记，但Xa - e被弱标记。蒙大拿果蝇和北方果蝇在Sd - f处的5S顺反子总数占比高于粗壮果蝇。美洲果蝇美洲亚种、美洲果蝇得克萨斯亚种和新墨西哥果蝇显示出2个标记位点；在Sd - f处未观察到标记，而Wc - j被弱标记，Xa - e被强标记，这与W - X区域中先前模式相反。美洲果蝇美洲亚种和粗壮果蝇之间的杂种在5S簇位点的染色体带型上没有差异，尽管它们的模式不同。另一方面，美洲果蝇得克萨斯亚种×粗壮果蝇在Xa - e区域的染色确实显示出差异。这些模式完全符合许多先前研究者推导的生物系统分类。
