Donaldson K, Hill I M, Beswick P H
Department of Biological Sciences, Napier University, Edinburgh, UK.
Exp Toxicol Pathol. 1995 Sep;47(4):229-31. doi: 10.1016/S0940-2993(11)80253-8.
Phagocyte-derived free radicals are considered to play a role in fibre-related pathology and the components of the lung lining fluid could modify the surface of fibres. Therefore we examined the ability of long amosite asbestos and a range of man-made fibres to stimulate release of superoxide anion from rat alveolar macrophages when they were in their native form (unopsonised) and opsonised by incubation in rat Immunoglobulin G. We also assessed the specific amount of opsonin adsorbed to each fibre type. In the uncoated form all of the fibres produced modest amounts of superoxide release from macrophages. When they were opsonised however there was an effect on stimulation of release of superoxide that was fibre-specific. Both MMVF21 and RCF 1 were dramatically enhanced in their ability to stimulate release and this was related to a high affinity of their surface for IgG. Code 100/475 and SiC were not substantially affected by opsonisation and this was reflected in their low affinity for IgG. Long amosite had low affinity for IgG but showed dramatic enhancement of capacity to stimulate superoxide release. These fibre-specific differences in the effect of a coating of material that is found in the lung lining points out the problems of interpretation of in vitro data and more work on this important area is warranted.
吞噬细胞衍生的自由基被认为在纤维相关病理学中起作用，并且肺内衬液的成分可能会改变纤维的表面。因此，我们研究了长纤维状青石棉和一系列人造纤维在天然形式（未调理）以及通过在大鼠免疫球蛋白G中孵育进行调理后，刺激大鼠肺泡巨噬细胞释放超氧阴离子的能力。我们还评估了吸附到每种纤维类型上的调理素的具体量。在未包被形式下，所有纤维都能使巨噬细胞释放少量超氧阴离子。然而，当它们被调理后，对超氧阴离子释放的刺激存在纤维特异性效应。MMVF21和RCF 1刺激释放的能力显著增强，这与它们表面对IgG的高亲和力有关。100/475号纤维和碳化硅受调理作用影响不大，这体现在它们对IgG的低亲和力上。长纤维状青石棉对IgG的亲和力较低，但刺激超氧阴离子释放的能力显著增强。肺内衬中发现的物质包被效应的这些纤维特异性差异指出了体外数据解释的问题，因此有必要在这一重要领域开展更多工作。
