Zurovsky Y, Eligal Z
Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Exp Toxicol Pathol. 1995 Sep;47(4):293-7. doi: 10.1016/S0940-2993(11)80263-0.
Previous studies have shown that the survival time of rats following binephrectomy is between 48 and 120 hours. The aim of the present study was to examine the assumption that the eating and drinking protocol before and after binephrectomy affects the survival time following surgery in male, female and young rats. In this study we used protocols which included various combinations of eating and drinking or their prevention before and after binephrectomy. Survival time was determined by examining the kinetics of death following binephrectomy. Examination of the azotemia and physiological condition of the rat was performed by testing BUN, creatinine, plasma Na and K concentrations as well as the hematocrit. All rats that had eaten and drunk before and after binephrectomy, in various combinations, died within 48 hours. Rats that did not eat or drink before and after binephrectomy died within 72 hours (p < 0.001). Rats that did not eat before and after the binephrectomy but had free access to drink died within 90 hours of the surgery (p < 0.001). From this study it can be concluded that the eating and drinking protocol before and after binephrectomy affects the survival time of male, female and young rats. The effect varies between a survival time of 48 and 90 hours following binephrectomy. A possible explanation for this effect is that the eating and drinking protocol affects the rate of azotemia formation.
以往的研究表明，大鼠双侧肾切除术后的存活时间在48至120小时之间。本研究的目的是检验这样一种假设，即双侧肾切除术前和术后的饮食方案会影响雄性、雌性和幼年大鼠术后的存活时间。在本研究中，我们采用了包括双侧肾切除术前和术后饮食或禁饮食的各种组合的方案。通过检查双侧肾切除术后的死亡动力学来确定存活时间。通过检测血尿素氮、肌酐、血浆钠和钾浓度以及血细胞比容来检查大鼠的氮质血症和生理状况。所有在双侧肾切除术前和术后以各种组合进食和饮水的大鼠均在48小时内死亡。双侧肾切除术前和术后不进食或不饮水的大鼠在72小时内死亡（p<0.001）。双侧肾切除术前和术后不进食但可自由饮水的大鼠在术后90小时内死亡（p<0.001）。从本研究可以得出结论，双侧肾切除术前和术后的饮食方案会影响雄性、雌性和幼年大鼠的存活时间。双侧肾切除术后存活时间在48至90小时之间变化。对此效应的一种可能解释是饮食方案会影响氮质血症的形成速率。
