Billings-Gagliardi S, Wolf M K
In Vitro. 1977 Jun;13(6):371-7. doi: 10.1007/BF02615097.
This paper describes a method for examination of living organotypic cultures of CNS with Nomarski differential interference-contrast optics. Cultures grown in Maximow assemblies, which promote the best differentiation of the tissue but are optically faulty, are transferred for Nomarski observation to a simple sandwich chamber which combines the optical perfection of the usual sandwich chamber with the flexibility and safeguarding of sterility characteristic of the Maximow assembly. Thus cultures can be transferred repeatedly between their maintenance and observation chambers. In the resulting microscopic images, it is possible to visualize delicate unmyelinated fibers, myelinated cell bodies and other features which are normally impossible to demonstrate in living cultures as well as to improve the images of other structures such as large neuronal perikarya and myelinated axons.
本文描述了一种利用诺马斯基微分干涉相差光学技术检查中枢神经系统（CNS）活组织型培养物的方法。在马克西莫夫培养装置中生长的培养物，虽能促进组织的最佳分化，但存在光学缺陷，将其转移至一个简单的三明治培养室进行诺马斯基观察，该培养室结合了普通三明治培养室的光学完美性以及马克西莫夫培养装置的灵活性和无菌保障特性。这样培养物就可以在其维持室和观察室之间反复转移。在所得的显微图像中，可以看到精细的无髓纤维、有髓细胞体以及其他通常在活培养物中无法显示的特征，同时还能改善其他结构的图像，如大型神经元胞体和有髓轴突。
