Whitworth I H, Brown R A, Doré C J, Anand P, Green C J, Terenghi G
Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, UK.
J Hand Surg Br. 1996 Aug;21(4):514-22. doi: 10.1016/s0266-7681(96)80058-1.
Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate (P < or = 0.007) and volume (P < or = 0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.
可溶性纤连蛋白和神经生长因子（NGF）置于硅胶管中时可促进轴突再生。我们研究了定向纤连蛋白垫结合和释放生物活性NGF的能力，以及使用浸渍NGF的纤连蛋白导管增强轴突切断术后轴突再生的可能性。使用荧光酶联免疫吸附测定法对NGF的释放进行定量，并通过神经元培养生物测定法确认其生物活性。采用免疫组织化学技术和计算机图像分析来评估轴突和雪旺细胞再生的速率和体积。与普通纤连蛋白导管相比，将NGF输送到损伤部位可使轴突和雪旺细胞再生的速率（P≤0.007）和体积（P≤0.004）增加。我们得出结论，浸渍NGF的纤连蛋白导管局部输送可增强轴突再生。
