Winkler B S, Simson V, Benner J
Invest Ophthalmol Vis Sci. 1977 Aug;16(8):766-8.
This study demonstrates a requirement for bicarbonate in retinal function. Omission of bicarbonate, with either phosphate or Tris used to maintain a constant pH, leads to significant changes in both the electroretinogram potentials and metabolism of the isolated rat retina. Electroretinograms in bicarbonate-free media show a selective loss of the b wave and a decline in the amplitudes of the a wave and Slow PIII. Substitution of phosphate for bicarbonate causes a 60 and 52% decrease in aerobic and anaerobic lactate production, respectively, but this substitution leads to a 27% increase in aerobic retinal ATP content. It is suggested that the electrical effects of bicarbonate-free media are due to a decrease in utilization of ATP below critical levels at critical sites.
本研究表明视网膜功能需要碳酸氢盐。用磷酸盐或Tris维持恒定pH值而省略碳酸氢盐，会导致分离的大鼠视网膜的视网膜电图电位和代谢发生显著变化。无碳酸氢盐培养基中的视网膜电图显示b波选择性丧失，a波和慢PIII振幅下降。用磷酸盐替代碳酸氢盐分别导致有氧和无氧乳酸产生减少60%和52%，但这种替代导致有氧视网膜ATP含量增加27%。有人认为，无碳酸氢盐培养基的电效应是由于关键部位ATP利用低于临界水平而降低所致。
