Raison S, Rousset C, Pujol J F, Weissmann D
Laboratoire de Neuropharmacologie Moléculaire, UCBL-CNRS, UMR 5542, Faculté de Médecine, Lyon, France.
J Neurochem. 1996 Nov;67(5):2124-33. doi: 10.1046/j.1471-4159.1996.67052124.x.
Tryptophan hydroxylase distribution was examined across the nuclei raphe dorsalis, medianus, and pontis of the adult rat, under basal conditions and 2 days after a single injection of p-chlorophenylalanine, an irreversible tryptophan hydroxylase inhibitor. Tryptophan hydroxylase-expressing cells were numbered in transverse sections processed for immunohistochemistry, and the area of tryptophan hydroxylase distribution was delineated in adjacent sections transferred onto nitrocellulose and processed for immunoautoradiography. Two distinct areas were visualized: an inner zone, corresponding to the area displaying tryptophan hydroxylase-immunoreactive cells (so-called somatic area), and an outer zone, here called perisomatic, devoid of perikarya yet rich in tryptophan hydroxylase-positive neuropil in the histological sections. After treatment with p-chlorophenylalanine, a significant decrease in the number of tryptophan hydroxylase-immunoreactive cells could be observed only in the rostral raphe dorsalis, particularly within its ventromedian and dorsomedian subdivisions. In all raphe nuclei, the topological reconstruction of the somatic area was not modified. Based on the densitometric measurements in the immunoautoradiographs, however, a dramatic decrease in the content, concentration, and volume of expression of tryptophan hydroxylase could be documented in the three raphe nuclei. Detailed analysis of these results led to the conclusion that (a) tryptophan hydroxylase expression is differentially regulated in different serotoninergic cell body subpopulations of the raphe, some of which are more sensitive to p-chlorophenylalanine, and (b) distribution of tryptophan hydroxylase protein is modified also in the somatodendritic area in all raphe nuclei.
在基础条件下以及单次注射对氯苯丙氨酸（一种不可逆的色氨酸羟化酶抑制剂）2天后，对成年大鼠背侧中缝核、中缝正中核和脑桥中缝核中的色氨酸羟化酶分布进行了检查。对经免疫组织化学处理的横切片中表达色氨酸羟化酶的细胞进行计数，并在转移到硝酸纤维素膜上并经免疫放射自显影处理的相邻切片中勾勒出色氨酸羟化酶分布区域。观察到两个不同的区域：一个内部区域，对应于显示色氨酸羟化酶免疫反应性细胞的区域（所谓的胞体区域），以及一个外部区域，此处称为胞体周围区域，在组织学切片中没有核周体，但富含色氨酸羟化酶阳性神经纤维网。在用对氯苯丙氨酸处理后，仅在吻侧背侧中缝核中，特别是在其腹内侧和背内侧亚区域中，观察到色氨酸羟化酶免疫反应性细胞数量显著减少。在所有中缝核中，胞体区域的拓扑重建未发生改变。然而，根据免疫放射自显影片中的光密度测量，在三个中缝核中可以记录到色氨酸羟化酶的含量、浓度和表达量显著下降。对这些结果的详细分析得出以下结论：（a）色氨酸羟化酶的表达在中缝不同的5-羟色胺能细胞体亚群中受到不同调节，其中一些对氯苯丙氨酸更敏感；（b）在所有中缝核的树突状区域中，色氨酸羟化酶蛋白的分布也发生了改变。
