Weissmann D, Chamba G, Debure L, Rousset C, Richard F, Maître M, Pujol J F
Laboratoire de Neuropharmacologie Moléculaire, C.N.R.S. U.M.R. 105, C.E.R.M.E.P. 59, Lyon, France.
Brain Res. 1990 Dec 17;536(1-2):46-55. doi: 10.1016/0006-8993(90)90007-x.
Distribution of tryptophan-5-hydroxylase (TpOH)-containing cells and TpOH protein tissue concentrations were evaluated in the nucleus raphe dorsalis (NRD) of rat brain by immunocytochemistry and direct transfer onto nitrocellulose filters of unfixed adjacent brain sections. This work has demonstrated that: (1) the direct transfer onto nitrocellulose filters could be easily used for the quantitative analysis of TpOH protein distribution; (2) the origin of the TpOH in this brain nucleus was preferentially cellular; (3) classical subdivisions, qualitatively defined from morphometric and topographic observations could be precisely described in terms of cellular density, tissue and cellular concentrations and turnover of TpOH protein. Such differences could imply a physiological control of TpOH gene expression in the serotoninergic neurons.
通过免疫细胞化学以及将未固定的相邻脑切片直接转移至硝酸纤维素滤膜上，对大鼠脑背侧中缝核（NRD）中含色氨酸-5-羟化酶（TpOH）的细胞分布和TpOH蛋白组织浓度进行了评估。这项研究表明：（1）直接转移至硝酸纤维素滤膜上可轻松用于TpOH蛋白分布的定量分析；（2）该脑核中TpOH的来源主要是细胞；（3）根据形态学和地形学观察定性定义的经典亚区，可以根据细胞密度、组织和细胞浓度以及TpOH蛋白的更新情况进行精确描述。这些差异可能意味着5-羟色胺能神经元中TpOH基因表达受到生理调控。
