Bekers O, Postma C, Fischer J C, Franck P F, Lombarts A J
Department of Clinical Chemistry and Haematology, Leyenburg Hospital, The Hague, The Netherlands.
Eur J Clin Chem Clin Biochem. 1996 Jul;34(7):561-3.
The well-known Kjeldahl method for the determination of faecal nitrogen is rather complex, time-consuming and expensive. Therefore, the use of near-infrared spectroscopy in determining the amount of nitrogen in faeces has been studied. To our knowledge we are the first to present the calibration equation for the determination of nitrogen with near-infrared spectroscopy. A good correlation (r = 0.96) was found between results from near-infrared spectroscopy and the Kjeldahl method. The imprecision of both methods was comparable. Once the rather laborious calibration has been performed, near-infrared spectroscopy is shown to be a very simple and rapid method for measuring nitrogen in faeces.
著名的凯氏定氮法用于测定粪便中的氮，该方法相当复杂、耗时且昂贵。因此，人们研究了使用近红外光谱法来测定粪便中的氮含量。据我们所知，我们是首个提出用近红外光谱法测定氮含量的校准方程的。近红外光谱法的结果与凯氏定氮法的结果之间存在良好的相关性（r = 0.96）。两种方法的不精密度相当。一旦完成了相当繁琐的校准，近红外光谱法就被证明是一种测量粪便中氮的非常简单且快速的方法。
