Benini L, Caliari S, Bonfante F, Guidi G C, Brentegani M T, Castellani G, Sembenini C, Bardelli E, Vantini I
Divisione di Gastroenterologia, Università di Verona, Italy.
Gut. 1992 Jun;33(6):749-52. doi: 10.1136/gut.33.6.749.
Chemical methods of measuring nitrogen in stools are complex, unpleasant, and therefore rarely performed. Recently, near infrared reflectance (NIRA) has been suggested for stool analysis. The aim of this study was to evaluate the possible application of this method in routine faecal nitrogen measurement. Nitrogen concentration and daily output were measured in the stools of 83 patients using NIRA and, for comparison, the Kjeldahl method. Nitrogen concentration and output ranged between 0.4-2.72 g% and 0.45-8.96 g/day respectively. Correlation coefficients (r), of 0.89 and 0.97 were found between the two methods for concentration and output respectively, and similar values were found in patients on enteral nutrition. Repeated measurements from the same stool collection, requiring only a few minutes, allowed homogenisation to be avoided. NIRA seems to be an easy, fast, and reliable alternative to chemical assays of nitrogen measurement in the management of patients with digestive disorders.
测量粪便中氮含量的化学方法复杂、令人不适,因此很少使用。最近,有人提出用近红外反射率(NIRA)进行粪便分析。本研究的目的是评估该方法在常规粪便氮含量测量中的可能应用。使用NIRA并与凯氏定氮法比较,测量了83例患者粪便中的氮浓度和日排出量。氮浓度和排出量分别在0.4 - 2.72 g%和0.45 - 8.96 g/天之间。两种方法测得的浓度和排出量的相关系数(r)分别为0.89和0.97,肠内营养患者也得到了相似的值。对同一份粪便样本进行重复测量,仅需几分钟,无需进行匀浆。在消化系统疾病患者的管理中,NIRA似乎是一种简便、快速且可靠的氮含量化学检测替代方法。