Li Y, Juul S E, Morris-Wiman J A, Calhoun D A, Christensen R D
Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610-0296, USA.
Pediatr Res. 1996 Sep;40(3):376-80. doi: 10.1203/00006450-199609000-00002.
Recombinant erythropoietin (rEpo) is an effective treatment for infants with the anemia of prematurity. rEpo was previously thought to act only on erythroid progenitor cells, but evidence now indicates that certain nonerythroid cells also express functional erythropoietin receptors (Epo-R). Such receptors have been observed on cells in the developing murine brain and spinal cord. The objective of this study was to determine whether Epo-R are expressed in the CNS of mid-trimester human fetuses. For this study, spinal cords were collected from five mid-trimester abortuses. RNA was extracted from the washed specimens, and the presence of Epo-R mRNA was sought by reverse transcription followed by polymerase chain reaction. Immunohistochemistry was then used to determine the anatomic location of the cells expressing Epo-R within the fetal spinal cord. The results showed that all fetal spinal cords tested contained Epo-R mRNA. The cells expressing Epo-R were radiating from the ependymal canal toward the anterior and posterior median sulci. We conclude that Epo-R are expressed on cells in the developing human CNS. Further studies are needed to determine whether they are clinically relevant in the premature infant.
重组促红细胞生成素（rEpo）是治疗早产贫血婴儿的有效方法。rEpo以前被认为仅作用于红系祖细胞，但现在有证据表明某些非红系细胞也表达功能性促红细胞生成素受体（Epo-R）。在发育中的小鼠脑和脊髓细胞上已观察到此类受体。本研究的目的是确定Epo-R是否在孕中期人类胎儿的中枢神经系统中表达。对于本研究，从五个孕中期流产胎儿获取脊髓。从洗涤后的标本中提取RNA，通过逆转录随后进行聚合酶链反应寻找Epo-R mRNA的存在。然后使用免疫组织化学来确定胎儿脊髓内表达Epo-R的细胞的解剖位置。结果显示，所有测试的胎儿脊髓都含有Epo-R mRNA。表达Epo-R的细胞从室管膜管向前后正中沟呈放射状分布。我们得出结论，Epo-R在发育中的人类中枢神经系统细胞上表达。需要进一步研究以确定它们在早产儿中是否具有临床相关性。
