Mukai H Y, Kojima H, Todokoro K, Tahara T, Kato T, Hasegawa Y, Kobayashi T, Ninomiya H, Nagasawa T, Abe T
Division of Hematology, University of Tsukuba, Ibaraki, Japan.
Thromb Haemost. 1996 Nov;76(5):675-8.
We assayed serum thrombopoietin (TPO) levels in amegakaryocytic thrombocytopenia (AMT) and immune thrombocytopenic purpura (ITP) patients by using a newly established enzyme-linked immunosorbent assay (ELISA). TPO levels in AMT patients were quite high (mean +/- SD = 13.7 +/- 11.2 fmoles/ml, n = 4), whereas those in ITP patients were only slightly higher (1.25 +/- 0.39, n = 12) than those of the healthy donors (0.55 +/- 0.2, n = 20). Furthermore, in ITP patients no correlation was observed between platelet counts and serum TPO levels (correlation coefficient = 0.14). We further assayed serum TPO levels sequentially during steroid treatment in patients with AMT and ITP. In one AMT patient serum TPO levels started to decrease in accordance with the increase of megakaryocyte counts, which preceded the increase in platelet counts. However, in ITP patients serum TPO levels did not change significantly throughout the course of the treatment despite the recovery of platelet counts. Based on these findings, we conclude that serum TPO levels may be regulated at least in part by megakaryocyte counts.
我们采用新建立的酶联免疫吸附测定法（ELISA）检测了无巨核细胞性血小板减少症（AMT）和免疫性血小板减少性紫癜（ITP）患者的血清血小板生成素（TPO）水平。AMT患者的TPO水平相当高（均值±标准差=13.7±11.2飞摩尔/毫升，n = 4），而ITP患者的TPO水平仅略高于健康供者（1.25±0.39，n = 12）（健康供者为0.55±0.2，n = 20）。此外，在ITP患者中，未观察到血小板计数与血清TPO水平之间存在相关性（相关系数=0.14）。我们进一步对AMT和ITP患者在类固醇治疗期间连续检测血清TPO水平。在一名AMT患者中，血清TPO水平随着巨核细胞计数的增加而开始下降，这早于血小板计数的增加。然而，在ITP患者中，尽管血小板计数恢复，但在整个治疗过程中血清TPO水平并未显著变化。基于这些发现，我们得出结论，血清TPO水平可能至少部分受巨核细胞计数的调节。
