Gupta S, Upadhayay R, Kanungo M S
Department of Zoology, Banaras Hindu University, Varanasi, India.
Biochem Mol Biol Int. 1996 Aug;39(5):887-94. doi: 10.1080/15216549600201032.
This study was directed at achieving an understanding of the mechanisms by which steroid hormones control the synthesis of vitellogenin (VTG) protein in the liver of the Japanese quail. Northern hybridization shows that administration of estradiol alone or with progesterone stimulates the synthesis of VTG mRNA. Gel mobility shift assay of DNA fragments containing the ERE and NF 1 shows that estradiol alone or with progesterone increases the levels of nuclear proteins that bind to these cis-acting elements of the promoter of the VTG gene. The cooperative effect of the two hormones seen at the level of expression of the VTG gene may be due to protein-protein interactions of trans-acting factors that bind to ERE and NF 1.
本研究旨在了解类固醇激素控制日本鹌鹑肝脏中卵黄蛋白原（VTG）合成的机制。Northern杂交显示，单独给予雌二醇或与孕酮一起给予会刺激VTG mRNA的合成。对含有雌激素反应元件（ERE）和核因子1（NF 1）的DNA片段进行凝胶迁移率变动分析表明，单独给予雌二醇或与孕酮一起给予会增加与VTG基因启动子这些顺式作用元件结合的核蛋白水平。在VTG基因表达水平上观察到的两种激素的协同作用可能是由于与ERE和NF 1结合的反式作用因子之间的蛋白质-蛋白质相互作用。
