Gupta S, Upadhyay R, Kanungo M S
Department of Zoology, Banaras Hindu University, Varanasi, India.
Mol Biol Rep. 1998 Nov;25(4):245-51. doi: 10.1023/a:1006997410211.
Alterations in the basal transcription rates of eukaryotic genes are believed to involve the binding of trans-acting factor(s) with specific DNA sequences in the promoter. We show here two interrelated events for the VTGII gene of the old, non-egg laying Japanese quail: alterations in the structure of the chromatin encompassing the gene, and binding of trans-acting factors to the promoter of the gene. Estradiol/progesterone alone or together cause alterations in the conformation of the chromatin of the promoter region of the gene. This may allow free access of nuclear protein(s) to the cis-acting elements, ERE, PRE and NF1, in the promoter of the gene and cause activation of transcription.
真核基因基础转录率的改变被认为涉及反式作用因子与启动子中特定DNA序列的结合。我们在此展示了老龄、不产蛋日本鹌鹑的VTGII基因的两个相互关联的事件：包含该基因的染色质结构的改变，以及反式作用因子与该基因启动子的结合。单独或共同使用雌二醇/孕酮会导致该基因启动子区域染色质构象的改变。这可能使核蛋白能够自由接近该基因启动子中的顺式作用元件ERE、PRE和NF1，并导致转录激活。
