Fischbarg J, Lim J J, Bourguet J
J Membr Biol. 1977 Jun 30;35(2):95-112. doi: 10.1007/BF01869942.
The rate of fluid transport across rabbit corneal endothelium has been measured with an automatic volumetric method. The present resolution of the procedure is 1-3 nanoliters, and intervals of measurement can be made as small as seconds. In the presence of glucose, oxidized glutathione (GSSG), and adenosine, the maximal rates were 6.2+/-1.0 microliter/hr cm2, and 8.2+/-0.8 microliter/hr cm2 if a large portion of the stroma was dissected away. In the presence of glucose and GSSG only, the rates were lower, namely 3.7+/-0.5 microliter/hr cm2. The rates consistently increased or decreased when adenosine was added or deleted, respectively, during given experiments. The stimulation of fluid transport by adenosine was in the order of 40-50%. The results raise the possibility that this transport mechanism might be subject to metabolic control.
采用自动容量法测量了兔角膜内皮细胞的液体转运速率。该方法目前的分辨率为1 - 3纳升，测量间隔可短至数秒。在葡萄糖、氧化型谷胱甘肽（GSSG）和腺苷存在的情况下，最大转运速率为6.2±1.0微升/小时·平方厘米；如果切除大部分基质，最大转运速率为8.2±0.8微升/小时·平方厘米。仅在葡萄糖和GSSG存在的情况下，转运速率较低，即3.7±0.5微升/小时·平方厘米。在给定实验中，分别添加或去除腺苷时，转运速率持续增加或降低。腺苷对液体转运的刺激作用约为40 - 50%。这些结果增加了这种转运机制可能受代谢控制的可能性。
