Ono S, Takahashi K, Kanda F, Fukuoka Y, Jinnai K, Kurisaki H, Mitake S, Inagaki T, Nagao K
Department of Neurology, Teikyo University School of Medicine, Ichiara Hospital, Chiba, Japan.
J Neurol Sci. 1996 Sep 1;140(1-2):96-100. doi: 10.1016/0022-510x(96)00107-4.
Intracytoplasmic inclusion bodies of the thalamus in eight patients with myotonic dystrophy (MyD) were studied immunohistochemically. The intracytoplasmic inclusion bodies of the thalamus (thalamic inclusions, TIs) were strongly immunostained with anti-ubiquitin antibody (Ab) and some of them were mildly stained with anti-microtubule associated protein 1 (MAP 1) and anti-MAP 2 antibodies. However, TIs did not react with any of the following: anti-neurofilament protein Ab, anti-tau Ab, anti-paired helical filament Ab, anti-tubulin Abs (alpha and beta), anti-neuron-specific enolase Ab, anti-glial fibrillary acidic protein Ab, anti-synaptophysin Ab, anti-myelin basic protein Ab, anti-actin Ab and anti-phosphorylated epitope of neurofilaments Ab. Thus, our study demonstrates the unique immunohistochemistry of TIs in MyD which differentiates them from other intracytoplasmic inclusions in various neurodegenerative disorders.
对8例强直性肌营养不良（MyD）患者丘脑的胞质内包涵体进行了免疫组织化学研究。丘脑的胞质内包涵体（丘脑包涵体，TIs）用抗泛素抗体（Ab）进行强免疫染色，其中一些用抗微管相关蛋白1（MAP 1）和抗MAP 2抗体进行轻度染色。然而，TIs与以下任何一种抗体均无反应：抗神经丝蛋白Ab、抗tau Ab、抗双螺旋丝Ab、抗微管蛋白Abs（α和β）、抗神经元特异性烯醇化酶Ab、抗胶质纤维酸性蛋白Ab、抗突触素Ab、抗髓鞘碱性蛋白Ab、抗肌动蛋白Ab和抗神经丝蛋白磷酸化表位Ab。因此，我们的研究证明了MyD中TIs独特的免疫组织化学，这使其与各种神经退行性疾病中的其他胞质内包涵体区分开来。
