Sharpe M E, Errington J
Sir William Dunn School of Pathology, University of Oxford, UK.
Mol Microbiol. 1996 Aug;21(3):501-9. doi: 10.1111/j.1365-2958.1996.tb02559.x.
During sporulation in Bacillus subtilis a small prespore cell is formed by an asymmetric cell division. Pre-spore chromosome partitioning occurs by a specialised mechanism in which septation precedes chromosome movement. We show that the spo0J gene is needed to specify the orientation of the chromosome at the time of polar division and to impose directionality on the subsequent transport of the remainder of the chromosome through the septum. Both phenotypes may arise by disruption of a centromere-like apparatus that anchors the or/C region of the prespore chromosome in the pole of the cell.
在枯草芽孢杆菌的孢子形成过程中，一个小的前芽孢细胞通过不对称细胞分裂形成。前芽孢染色体的分配通过一种特殊机制进行，其中隔膜形成先于染色体移动。我们发现，spo0J基因对于在极性分裂时确定染色体的方向以及在随后染色体其余部分通过隔膜的运输过程中赋予方向性是必需的。这两种表型可能是由于一种类似着丝粒的装置被破坏所致，该装置将前芽孢染色体的or/C区域锚定在细胞的极部。
