Alenfall J, Batra S
Department of Pharmacology, University of Lund, Sweden.
Biochem Pharmacol. 1996 Apr 26;51(8):1009-13. doi: 10.1016/0006-2952(95)02400-x.
Photoaffinity labeling of peripheral benzodiazepine receptors (PBZr) in mitochondrial and microsomal fractions from AT-1, H, and G Dunning R-3327 tumor sublines was performed using the photoaffinity ligand 3H-PK 14105. Subsequent sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed specific labeling of a 17 kDa protein in the microsomal fractions from these tumors. In the mitochondrial fractions, however, two bands with the apparent molecular mass of 17 and 18 kDa in AT-1 and H tumors were identified, whereas only the 17 kDa band was present in G tumors. Using another ligand, 3H-flunitrazepam, to photoaffinity label PBZr, proteins with molecular masses of 17, 30, 42, and 48 kDa were identified. These data suggest the possibility of different subclasses of PBZrs in Dunning prostatic tumors.
使用光亲和配体3H-PK 14105对来自AT-1、H和G邓宁R-3327肿瘤亚系的线粒体和微粒体部分中的外周苯二氮䓬受体（PBZr）进行光亲和标记。随后的十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）显示，这些肿瘤的微粒体部分中有一个17 kDa蛋白的特异性标记。然而，在线粒体部分中，AT-1和H肿瘤中鉴定出两条表观分子量为17 kDa和18 kDa的条带，而G肿瘤中仅存在17 kDa的条带。使用另一种配体3H-氟硝西泮对PBZr进行光亲和标记，鉴定出分子量为17、30、42和48 kDa的蛋白质。这些数据表明邓宁前列腺肿瘤中存在不同亚类PBZr的可能性。
