Sato I, Himi T, Murota S
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Neurosci Lett. 1996 Feb 16;205(1):45-8. doi: 10.1016/0304-3940(96)12377-6.
We examined the inducible form of nitric oxide synthase (iNOS) activity in cerebellar primary cultures enriched with granule neurons. Treatment with lipopolysaccharide (LPS) for 24 h was performed in two different cultures in which the survival of neurons was controlled by the levels of extracellular K+. Treatment of the granule neurons with the contaminating non-neuronal cells (< 5%) in a high K+ (25 mM) -containing culture medium with LPS induced four-fold NOS activity compared to that of the control, and the induced NOS activity was calcium-independent. LPS did not induce the NOS activity at all in the contaminating non-neuronal cells alone which were obtained by eliminating all the granule neurons from the culture by lowering the K+ concentration (5 mM). We conclude that the LPS-activated granule neurons can express iNOS activity and that this induction is not attributable to the contaminating nonneuronal cells.
我们检测了富含颗粒神经元的小脑原代培养物中一氧化氮合酶（iNOS）的诱导型活性。在两种不同的培养物中用脂多糖（LPS）处理24小时，其中神经元的存活由细胞外K +水平控制。在含有高K +（25 mM）的培养基中用污染的非神经元细胞（<5％）处理颗粒神经元并加入LPS，与对照相比，诱导的NOS活性增加了四倍，并且诱导的NOS活性不依赖于钙。单独在通过降低K +浓度（5 mM）从培养物中消除所有颗粒神经元而获得的污染非神经元细胞中，LPS根本不诱导NOS活性。我们得出结论，LPS激活的颗粒神经元可以表达iNOS活性，并且这种诱导不归因于污染的非神经元细胞。
