Sinakevitch I G, Geffard M, Pelhate M, Lapied B
Laboratoire de Neurophysiologie, CNRS ERS 108, Université d'Angers, France.
J Comp Neurol. 1996 Mar 25;367(1):147-63. doi: 10.1002/(SICI)1096-9861(19960325)367:1<147::AID-CNE9>3.0.CO;2-3.
The morphology of the Dorsal Unpaired Median (DUM) neurones in the Terminal Abdominal Ganglion (TAG) of the adult male cockroach Periplaneta americana were described based on wholemount preparations and paraffin sections and by using anterograde and retrograde cobalt mapping, octopamine-like immunohistochemistry, and double immunofluorescence technique with both conjugated gamma-aminobutyric acid (GABA) and octopamine antisera. Among 60 +/- 6 neurones with large somata (diameter 40 to 60 microns) on the dorsal midline surface of the TAG that were stained with toluidine blue, about 36 efferent DUM neurones exhibited octopamine-like immunoreactivity. The DUM neurones were arranged in three clusters (anterior, median and posterior) corresponding to the 7th-11th abdominal ganglia of the fused TAG. Anterior efferent DUM neurones with one, two, and four pairs of lateral neurites entered segmental nerves VIIB; VIIB and phallic nerves; IXB and phallic nerves; VIIIA, IXA, X, and IX, respectively. Three octopamine-like immunoreactive DUM neurones innervating heart chambers via segmental nerves (VIIA, VIIIA, and IXA) in the last abdominal segments occurred within abdominal ganglia 7, 8, and 9. Together with octopamine-like immunoreactive efferent DUM neurones, GABA-like immunoreactive dorsal midline neurones with small somata (10 to 20 microns) also occurred within the median group. The spatial distribution of DUM neurones in the TAG suggested that they had their origins in the median neuroblast, as for DUM neurones in the grasshopper.
基于整装标本和石蜡切片，并运用顺行和逆行钴标记法、章鱼胺样免疫组织化学以及结合了γ-氨基丁酸（GABA）和章鱼胺抗血清的双重免疫荧光技术，对成年雄性美洲大蠊终腹神经节（TAG）中背侧不成对中间神经元（DUM）的形态进行了描述。在TAG背中线表面60±6个经甲苯胺蓝染色的大细胞体（直径40至60微米）神经元中，约36个传出DUM神经元呈现出章鱼胺样免疫反应性。DUM神经元排列成三个簇（前部、中部和后部），对应于融合TAG的第7至11腹神经节。具有一对、两对和四对侧神经突的前部传出DUM神经元分别进入第VIIB节段神经；VIIB节段神经和阴茎神经；IXB节段神经和阴茎神经；VIIIA、IXA、X和IX节段神经。在最后腹节中，通过节段神经（VIIA、VIIIA和IXA）支配心室的三个章鱼胺样免疫反应性DUM神经元分别位于第7、8和9腹神经节内。与章鱼胺样免疫反应性传出DUM神经元一起，中部组内也存在具有小细胞体（10至20微米）的GABA样免疫反应性背中线神经元。TAG中DUM神经元的空间分布表明，它们与蝗虫中的DUM神经元一样，起源于中间神经母细胞。
