Kato S, Morita T, Hori T, Kato M, Hirano A, Herz F, Ohama E
Division of Neuropathology, Tottori University.
Noshuyo Byori. 1995;12(2):125-32.
This retrospective immunohistochemical study compares the expression of five stress-response (heat-shock) proteins (srp's) [srp 90, srp 72, srp 27, alpha B-crystallin and ubiquitin], p53 protein and proliferating cell nuclear antigen (PCNA) in 118 primary brain tumors and 21 carcinoma metastases to the central nervous system. Serial sections of formalin-fixed, paraffin-embedded tissues were used. Most astrocytomas (9/13), ependymomas (5/5), glioblastoma multiforme (GBM) (11/12), schwannomas (19/21), meningiomas (22/23) and breast carcinoma metastases (Br-Mt) (9/10), and some medulloblastomas (5/15), primitive neuroectodermal tumors (PNETs) (5/11), pituitary adenomas (4/7) and lung carcinoma metastases (6/11), but none of 10 oligodendrogliomas had tumor cells that expressed one or more (up to five) srp's. The percentage of tumors with p53-positive cells was variable; the proportion was highest among srp-expressing GBMs (mean: 16.1%) and Br-Mts (mean: 15.3%). The mean PCNA-labeling index (LI) also varied, ranging from 1.2% in the group of pituitary adenomas to 24.5% in Br-Mts, with GBMs (20.4%) and medulloblastomas (18.4%) approaching the latter value. PCNA-LI was higher in the astrocytomas, GBMs, medulloblastomas and PNETs that expressed srp's than in those did not. A high proportion of p53-positive cells (31.3 to 59.0%) and the highest PCNA-LIs (41.0 to 49.0%) were seen in two GBMs and one Br-Mt that expressed all five srp's. We conclude that primary and metastatic tumors of the brain produce one or more stress-related proteins, and that a variable proportion of the tumor cells have immunohistochemically-detectable p53, the expression of which may depend, at least in part, on the growth potential of a given tumor.
这项回顾性免疫组织化学研究比较了118例原发性脑肿瘤和21例中枢神经系统癌转移瘤中五种应激反应（热休克）蛋白（SRP's）[SRP 90、SRP 72、SRP 27、αB-晶状体蛋白和泛素]、p53蛋白和增殖细胞核抗原（PCNA）的表达情况。使用的是福尔马林固定、石蜡包埋组织的连续切片。大多数星形细胞瘤（9/13）、室管膜瘤（5/5）、多形性胶质母细胞瘤（GBM）（11/12）、神经鞘瘤（19/21）、脑膜瘤（22/23）和乳腺癌转移瘤（Br-Mt）（9/10），以及一些髓母细胞瘤（5/15）、原始神经外胚层肿瘤（PNETs）（5/11）、垂体腺瘤（4/7）和肺癌转移瘤（6/11），但10例少突胶质细胞瘤中均无表达一种或多种（最多五种）SRP's的肿瘤细胞。p53阳性细胞的肿瘤百分比各不相同；在表达SRP's的GBM中比例最高（平均：16.1%），在Br-Mt中次之（平均：15.3%）。平均PCNA标记指数（LI）也有所不同，范围从垂体腺瘤组的1.2%到Br-Mt组的24.5%，GBM（20.4%）和髓母细胞瘤（18.4%）接近后者的值。表达SRP's的星形细胞瘤、GBM、髓母细胞瘤和PNETs中的PCNA-LI高于不表达的。在表达所有五种SRP's的两个GBM和一个Br-Mt中，观察到高比例的p53阳性细胞（31.3%至59.0%）和最高的PCNA-LI（41.0%至49.0%）。我们得出结论，原发性和转移性脑肿瘤会产生一种或多种与应激相关的蛋白，并且可变比例的肿瘤细胞具有免疫组织化学可检测到的p53，其表达可能至少部分取决于特定肿瘤的生长潜能。
