Balíková M
Institute for Toxicology and Forensic Chemistry, Charles University, Prague, Czech Republic.
Sb Lek. 1994;95(4):339-45.
During four years experience high performance liquid chromatography (HPLC) with photodiode array detection has been proved to be the demanded method of systematic toxicological analysis (STA) for unknown drugs in biological sample because of separation efficiency, sensitivity, flexibility and identification potential. HPLC can be an easy way of quantitation as well. Ultraviolet spectra acquired with Waters 990+ photodiode array detector together with retention data are used to identify unknown or suspected drugs and metabolites in various biological material often after basic TLC examination of urine. At present spectrum library used for comparison involves 180 spectra of standards in acid and neutral media. Various sample preparation procedures have been tested and till now eight isocratic liquid chromatographic systems have been used routinely for sensitive screening in small samples, identification and/or quantitative assays of drug groups. These analytical systems are suitable for toxicological examinations of forensic cases, acute poisonings, drug abuse. They are convenient to subsequent monitoring of serum drug levels during treatment of an intoxication as well.
在四年的经验中，配备光电二极管阵列检测的高效液相色谱法（HPLC）已被证明是生物样品中未知药物系统毒理学分析（STA）所需的方法，这是由于其分离效率、灵敏度、灵活性和鉴定潜力。HPLC也是一种简便的定量方法。使用Waters 990+光电二极管阵列检测器获得的紫外光谱以及保留数据，通常在对尿液进行基本的薄层色谱（TLC）检查后，用于鉴定各种生物材料中的未知或疑似药物及代谢物。目前用于比较的光谱库包含180种酸和中性介质中标准品的光谱。已经测试了各种样品制备程序，到目前为止，八个等度液相色谱系统已常规用于小样品的灵敏筛选、药物组的鉴定和/或定量分析。这些分析系统适用于法医案件、急性中毒、药物滥用的毒理学检查。它们也便于在中毒治疗期间对血清药物水平进行后续监测。
