Nagyová A, Ginter E
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Physiol Res. 1995;44(5):301-5.
Chlorophenols, mainly used as biocides, are compounds with a wide spectrum of toxic effects including teratogenic and carcinogenic actions. In this study, the effects of 2,4-dichlorophenol (2,4-DCP) on hepatic microsomal cytochrome P-450, NADPH-cytochrome c reductase activity, liver ascorbic acid (AA) and glutathione (GSH) content were studied in guinea-pigs with a low (2 mg/day/animal) or a high (50 mg/day/animal) ascorbic acid intake. The high AA intake significantly increased liver AA and GSH levels. There was a clear-cut correlation between liver AA and GSH levels. Administration of 2,4-DCP significantly decreased cytochrome P-450 and NADPH-cytochrome c reductase activity in hepatic microsomes isolated from guinea-pigs with the low AA intake. Such a reduction was not observed in intoxicated guinea-pigs with the high AA intake. The results suggest that AA can play a protective role in 2,4-DCP toxicity.
氯酚主要用作杀生剂，是一类具有广泛毒性作用的化合物，包括致畸和致癌作用。在本研究中，研究了低剂量（2毫克/天/只动物）或高剂量（50毫克/天/只动物）摄入抗坏血酸的豚鼠中，2,4-二氯酚（2,4-DCP）对肝微粒体细胞色素P-450、NADPH-细胞色素c还原酶活性、肝脏抗坏血酸（AA）和谷胱甘肽（GSH）含量的影响。高抗坏血酸摄入量显著提高了肝脏抗坏血酸和谷胱甘肽水平。肝脏抗坏血酸和谷胱甘肽水平之间存在明显的相关性。给予2,4-DCP显著降低了低抗坏血酸摄入量的豚鼠肝脏微粒体中的细胞色素P-450和NADPH-细胞色素c还原酶活性。在高抗坏血酸摄入量的中毒豚鼠中未观察到这种降低。结果表明，抗坏血酸可以在2,4-DCP毒性中发挥保护作用。
