Tanimoto Y, Ichikawa Y, Yasuda Y, Tochikubo K
Department of Microbiology, Nagoya City University Medical School, Japan.
FEMS Microbiol Lett. 1996 Feb 15;136(2):151-6. doi: 10.1111/j.1574-6968.1996.tb08041.x.
Gramicidin S, dissolved in ethanol, penetrated into the inside of the dormant spores of Bacillus subtilis, had a partial inhibitory effect on L-alanine-initiated germination and completely inhibited their outgrowth and vegetative growth. The activity of particulate NADH oxidase of the antibiotic-treated dormant spores was also influenced significantly. Abnormal morphological changes were observed in germinated spores from gramicidin S-treated dormant spores. An immunoelectron microscopy method with colloidal gold-IgG complex showed that the penetration site of gramicidin S inside dormant spores was mainly the core region. These facts suggest that gramicidin S induces the damage of not only the outer membrane-spore coat complex but also the inner membrane surrounding the spore protoplast, and is able to penetrate into the core region of B. subtilis dormant spores.
溶解于乙醇中的短杆菌肽S可穿透枯草芽孢杆菌休眠孢子内部，对L-丙氨酸引发的萌发有部分抑制作用，并完全抑制其发芽和营养生长。经抗生素处理的休眠孢子的颗粒状NADH氧化酶活性也受到显著影响。在经短杆菌肽S处理的休眠孢子萌发的孢子中观察到异常形态变化。用胶体金-IgG复合物的免疫电子显微镜方法表明，短杆菌肽S在休眠孢子内部的穿透部位主要是核心区域。这些事实表明，短杆菌肽S不仅会诱导外膜-芽孢衣复合物受损，还会损伤围绕孢子原生质体的内膜，并且能够穿透枯草芽孢杆菌休眠孢子的核心区域。
