Fujita Y, Yasuda Y, Tochikubo K
Department of Microbiology, Nagoya City University Medical School, Aichi.
Microbiol Immunol. 1990;34(12):1013-23. doi: 10.1111/j.1348-0421.1990.tb01523.x.
The penetration of gentamicin and polymyxin B into the inside of Bacillus subtilis spores was examined by an immunoelectron microscopy method with colloidal gold--immunoglobulin G (IgG) complex. The colloidal gold particles were located predominantly in the coat region of both gentamicin-treated and polymyxin B-treated spores and were hardly observed in the other regions, i.e., the cortex and core regions. When these antibiotic-treated spores were subsequently treated with CaCl2, the number of gold particles bound to the coat region was greatly decreased. These results suggest that these two antibiotics are able to penetrate into the spore coat but not into the cortex or core, that is, the primary permeability barrier to them exists between the coat and the cortex regions.
采用胶体金-免疫球蛋白G（IgG）复合物免疫电子显微镜法，检测庆大霉素和多粘菌素B进入枯草芽孢杆菌芽孢内部的情况。胶体金颗粒主要位于经庆大霉素处理和经多粘菌素B处理的芽孢的外壳区域，而在其他区域，即皮层和核心区域几乎未观察到。当这些经抗生素处理的芽孢随后用氯化钙处理时，与外壳区域结合的金颗粒数量大大减少。这些结果表明，这两种抗生素能够穿透芽孢外壳，但不能穿透皮层或核心，也就是说，它们的主要渗透屏障存在于外壳和皮层区域之间。
