Ohlsson K, Linder C, Lundberg E, Axelsson L
Department of Surgical Pathophysiology, University of Lund, Malmö University Hospital, Sweden.
Scand J Clin Lab Invest. 1996 Aug;56(5):461-70. doi: 10.3109/00365519609088801.
Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNF alpha) was also measured with a bioassay. TNF alpha production and soluble TNF alpha receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNF alpha far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (IL1-ra), compared with interleukin-1 beta (IL-1 beta), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-1 beta was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.
采用酶联免疫吸附测定法，检测经调理酵母细胞或脂多糖刺激的外周血单核细胞和多形核细胞中促炎和抗炎细胞因子、中性粒细胞弹性蛋白酶及分泌型白细胞蛋白酶抑制剂（SLPI）的释放情况及细胞内含量。还通过生物测定法检测肿瘤坏死因子α（TNFα）。在两种细胞群体环境中均证实有TNFα产生及可溶性TNFα受体I（sTNF RI）。生物测定法显示的TNFα水平远低于酶联免疫吸附测定法检测到的水平。发现在受刺激细胞环境中，细胞因子及其抑制剂的总体分泌有利于抗炎平衡。与白细胞介素-1β（IL-1β）相比，白细胞介素-1受体拮抗剂（IL1-ra）在两种细胞类型的分泌物中均占主导，分别过量100至1000倍。大部分翻译后的IL-1β未分泌，而是与细胞区室相关。与脂多糖（LPS）刺激不同，调理酵母细胞刺激中性粒细胞大量释放弹性蛋白酶。
