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通过与富含腺嘌呤 - 胸腺嘧啶的DNA特异性结合的物质对大鼠进行荧光逆行神经元标记。

Fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich DNA.

作者信息

Bentivoglio M, Kuypers H G, Catsman-Berrevoets C E, Dann O

出版信息

Neurosci Lett. 1979 May;12(2-3):235-40. doi: 10.1016/0304-3940(79)96068-3.

DOI:10.1016/0304-3940(79)96068-3
PMID:88697
Abstract

Six fluorescent substances, binding specifically to adenine-thymine rich DNA, were injected in rat caudate-putamen. This resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe. Two of these substances, i.e. "True Blue' and 'Granular Blue', give a very striking blue fluorescent retrograde neuronal labeling. Mid-thoracic spinal injections of these two substances in rat also resulted in a pronounced retrograde fluorescent labeling of neurons of the descending brainstem pathways and of neurons in the sensori-motor cortex.

摘要

将六种能特异性结合富含腺嘌呤 - 胸腺嘧啶的DNA的荧光物质注入大鼠尾状核 - 壳核。这导致了轴突逆行运输以及中央 - 中脑束旁核、黑质和中缝背核中神经元的荧光逆行标记。其中两种物质,即“真蓝”和“颗粒蓝”,产生了非常显著的蓝色荧光逆行神经元标记。在大鼠胸段中部脊髓注射这两种物质也导致了下行脑干通路神经元和感觉运动皮层神经元的明显逆行荧光标记。

相似文献

1
Fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich DNA.通过与富含腺嘌呤 - 胸腺嘧啶的DNA特异性结合的物质对大鼠进行荧光逆行神经元标记。
Neurosci Lett. 1979 May;12(2-3):235-40. doi: 10.1016/0304-3940(79)96068-3.
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Dorsal raphe cells with collateral projections to the caudate-putamen and substantia nigra: a fluorescent retrograde double labeling study in the rat.向尾状核-壳核和黑质发出侧支投射的中缝背核细胞:大鼠荧光逆行双重标记研究
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The organization of the efferent projections of the substantia nigra in the rat. A retrograde fluorescent double labeling study.
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Simultaneous use of retrograde fluorescent tracers and fluorescence histochemistry for convenient and precise mapping of monoaminergic projections and collateral arrangements in the CNS.同时使用逆行荧光示踪剂和荧光组织化学技术,以便在中枢神经系统中方便、精确地绘制单胺能投射和侧支排列图谱。
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A method for tracing biochemically defined pathways in the central nervous system using combined fluorescence retrograde transport and immunohistochemical techniques.一种利用荧光逆行运输和免疫组织化学技术相结合来追踪中枢神经系统中生物化学定义通路的方法。
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Fluorometric comparisons of the retrograde axonal transport of true blue and diamidino yellow from the rat caudate-putamen.大鼠尾状核-壳核中真蓝和双脒基黄逆行轴突运输的荧光比较。
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Collaterals of rubrospinal neurons to the cerebellum in rat. A retrograde fluorescent double labeling study.大鼠红核脊髓神经元至小脑的侧支:逆行荧光双重标记研究
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Simultaneous fluorescent retrograde axonal tracing and immunofluorescent characterization of neurons.
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Selective retrograde labeling indicating the transmitter of neuronal pathways.选择性逆行标记显示神经通路的递质。
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Brain Res. 1981 Apr 27;211(1):15-36. doi: 10.1016/0006-8993(81)90064-0.

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