Qiao M, Saris P E
Institute of Biotechnology, University of Helsinki, Finland.
FEMS Microbiol Lett. 1996 Oct 15;144(1):89-93. doi: 10.1111/j.1574-6968.1996.tb08513.x.
The biosynthesis, immunity and regulation of nisin, a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis, is encoded by two gene clusters, nisA/ZBTCIPRK and nisFEG. The mutant strain LAC46 with a deletion in the translocator gene nisT could not secrete nisin but nisin activity was detected from cell lysates. The nisT mutation was complemented by a NisT-expression plasmid resulting in restored capacity to secrete nisin. These results demonstrate that NisT is the transport protein dedicated to translocate nisin and that dehydration and lanthionine formation in nisin maturation can occur independently of transport.
乳酸乳球菌产生的含羊毛硫氨酸抗菌肽乳链菌肽的生物合成、免疫和调控由两个基因簇nisA/ZBTCIPRK和nisFEG编码。转运体基因nisT缺失的突变菌株LAC46不能分泌乳链菌肽，但从细胞裂解物中检测到了乳链菌肽活性。nisT突变通过NisT表达质粒得到互补，从而恢复了分泌乳链菌肽的能力。这些结果表明，NisT是专门负责转运乳链菌肽的转运蛋白，并且乳链菌肽成熟过程中的脱水和羊毛硫氨酸形成可以独立于转运发生。
