Baskin L S, Hayward S W, Young P F, Cunha G R
Department of Urology, University of California, San Francisco 94143-0738, USA.
Acta Anat (Basel). 1996;155(3):163-71. doi: 10.1159/000147801.
The sequential expression of smooth muscle and epithelial markers in the rat bladder has been defined. Smooth muscle differentiation, based on cell morphology and immunohistochemical localization of smooth muscle alpha-actin, myosin, vinculin, desmin, vimentin and laminin, begins at 16 days of gestation (birth = 22 days) in the rat bladder. Smooth muscle cell differentiation begins in the periphery of the bladder mesenchyme immediately subadjacent to the serosa and continues toward the urothelium. The cytokeratins 5, 7, 8, 14, 18, and 19 are expressed in the bladder epithelium as a function of developmental age with cytokeratin 7, 8, 18, and 19 being coexpressed at 15 days' gestation followed by cytokeratin 5 at 17 days' gestation and cytokeratin 14 in the newborn bladder. An understanding of the ontogeny of both smooth muscle and epithelial differentiation markers in the normal bladder will allow us to study events that occur in the diseased bladder.
大鼠膀胱中平滑肌和上皮标志物的顺序表达已得到明确。基于细胞形态以及平滑肌α-肌动蛋白、肌球蛋白、纽蛋白、结蛋白、波形蛋白和层粘连蛋白的免疫组织化学定位，平滑肌分化在大鼠膀胱妊娠16天（出生为22天）时开始。平滑肌细胞分化始于紧邻浆膜的膀胱间充质周边，并朝着尿路上皮方向持续进行。细胞角蛋白5、7、8、14、18和19在膀胱上皮中的表达随发育年龄而变化，细胞角蛋白7、8、18和19在妊娠15天时共同表达，随后在妊娠17天时出现细胞角蛋白5，新生膀胱中出现细胞角蛋白14。了解正常膀胱中平滑肌和上皮分化标志物的个体发生情况将有助于我们研究患病膀胱中发生的事件。
