Burrone O, Algranati I D
Mol Cell Biochem. 1977 Jul 5;16(2):105-10. doi: 10.1007/BF01732050.
The ribosomal profiles in lysates from resting and phytohemagglutinin stimulated human lymphocytes have been analyzed by sucrose gradient centrifugation. The percentage of polyribosomes increased during lymphocyte transformation reaching a maximal value of 60 to 70% of the total ribosomes after 72 hours of mitogen addition. This time period coincides with maximal in vivo protein synthesis. On the other hand, in nonstimulated lymphocytes, about 25% of the ribosomal particles appeared as aggregates, independently of the incubation period. Experiments performed with homologous cell free systems containing ribosomes and supernatant fluids prepared from unstimulated or activated lymphocytes demonstrate that the mixtures containing both components from stimulated lymphocytes are several fold more active in polypeptide synthesis than the systems which contain ribosomal particles and cell sap from resting cells. Assays carried out with mixtures combining the components from both sources indicate that the increased activity depends on ribosomes as well as on the supernatant fractions.
已通过蔗糖梯度离心法分析了来自静息和经植物血凝素刺激的人淋巴细胞裂解物中的核糖体图谱。在淋巴细胞转化过程中，多核糖体的百分比增加，在添加促有丝分裂原72小时后达到总核糖体的60%至70%的最大值。这个时间段与体内最大蛋白质合成相吻合。另一方面，在未刺激的淋巴细胞中，约25%的核糖体颗粒呈现为聚集体，与孵育时间无关。用含有核糖体和从未刺激或活化的淋巴细胞制备的上清液的同源无细胞系统进行的实验表明，含有来自刺激淋巴细胞的两种成分的混合物在多肽合成中的活性比含有来自静息细胞的核糖体颗粒和细胞液的系统高几倍。对结合了两种来源成分的混合物进行的测定表明，活性增加既取决于核糖体，也取决于上清液部分。
