Berger N A, Skinner A M
J Cell Biol. 1974 Apr;61(1):45-55. doi: 10.1083/jcb.61.1.45.
Lymphocyte cultures from all normal human adults are stimulated by zinc ions to increase DNA and RNA synthesis and undergo blast transformation. Optimal stimulation occurs at 0.1 mM Zn(++). Examination of the effects of other divalent cations reveals that 0.01 mM Hg(++) also stimulates lymphocyte DNA synthesis. Ca(++) and Mg(++) do not affect DNA synthesis in this culture system, while Mn(++), Co(++), Cd(++), Cu(++), and Ni(++) at concentrations of 10(-7)-10(-3) M are inhibitory. DNA and RNA synthesis and blast transformation begin to increase after cultures are incubated for 2-3 days with Zn(++) and these processes reach a maximum rate after 6 days. The increase in Zn(++)-stimulated lymphocyte DNA synthesis is prevented by rendering cells incapable of DNA-dependent RNA synthesis with actinomycin D or by blocking protein synthesis with cycloheximide or puromycin. Zn(++)-stimulated DNA synthesis is also partially inhibited by 5'-AMP and chloramphenicol. Zn(++) must be present for the entire 6-day culture period to produce maximum stimulation of DNA synthesis. In contrast to its ability to independently stimulate DNA synthesis, 0.1 mM Zn(++) inhibits DNA synthesis in phytohemagglutinin-stimulated lymphocytes and L1210 lymphoblasts.
所有正常成年人的淋巴细胞培养物都会受到锌离子的刺激,从而增加DNA和RNA的合成,并发生原始细胞转化。在0.1 mM Zn(++)时可出现最佳刺激效果。对其他二价阳离子作用的研究表明,0.01 mM Hg(++)也能刺激淋巴细胞DNA的合成。在该培养系统中,Ca(++)和Mg(++)不影响DNA的合成,而浓度为10(-7)-10(-3) M的Mn(++)、Co(++)、Cd(++)、Cu(++)和Ni(++)具有抑制作用。用Zn(++)培养细胞2-3天后,DNA和RNA的合成以及原始细胞转化开始增加,这些过程在6天后达到最大速率。放线菌素D使细胞无法进行依赖DNA的RNA合成,或用环己酰亚胺或嘌呤霉素阻断蛋白质合成,均可阻止Zn(++)刺激的淋巴细胞DNA合成增加。5'-AMP和氯霉素也可部分抑制Zn(++)刺激的DNA合成。在整个6天的培养期内都必须存在Zn(++),才能对DNA合成产生最大刺激。与它独立刺激DNA合成的能力相反,0.1 mM Zn(++)可抑制植物血凝素刺激的淋巴细胞和L1210成淋巴细胞中的DNA合成。